Li Wang

University of Montreal
N cycling genes expression in canola root and rhizosphere in different agricultural rotation systems

This research targets canola, one of the most widely grown Canadian crops and aims to enhance canola productivity and reduce fertilizer use in agricultural rotation systems of canola, wheat, lentil and chem-fallow through manipulation of the microbiome. Nitrification and denitrification lead to considerable losses of nitrogen fertilizer through leaching and emissions of the greenhouse gas N2O, and biological N2-fixation brings in free N. Understanding the relationship between the canola root microbiome and the expression of the microbial genes nifH, amoA, amoB, nxrA, nirS, nirK, and nosZ, which control these processes is crucial to sustainably intensify canola production. In Swift Current, 3x5 factorial design in RCBD with 4 blocks = 60 plots. There are 3 levels of previous rotation crop (lentil, wheat, and chem-fallow), followed by 5 levels of Brassicaceae (canola (Brassica napus and B. juncea) /oil seeds (Camelina sp.) and Brassica carinata and Sinapis alba). We will analyze the expression of genes involved in the processes of biological N2-fixation, nitrification, and denitrification in canola root and rhizosphere of the Brassicaceae crops at the full bloom stage.